NAD+
Nicotinamide Adenine Dinucleotide (NAD+) is a coenzyme found in every cell that is essential for metabolism, energy production, DNA repair, and activation of sirtuins — the longevity genes. NAD+ levels decline significantly with age, contributing to cellular dysfunction, fatigue, cognitive decline, and aging. Supplementation via subcutaneous injection or IV provides a direct boost to NAD+ levels, supporting mitochondrial function, enhancing mental clarity, improving energy, and promoting healthy aging. It is a cornerstone of modern longevity protocols.
Frequency
Subcutaneous: daily. IV: 1-2x per week or per protocol.
Cycle Length
Can be used long-term. Many use continuously as an anti-aging protocol.
Route
Intramuscular
Research Level
Established
Goals
Dosing Protocol
50-150 mg daily, or higher doses 2x per week. Single dose not to exceed 250 mg. Intramuscular injection. NAD+ vials are typically pre-dissolved — draw and inject per volume instructions on vial.
Reconstitution
Typically supplied as a pre-mixed solution. If lyophilized, add bacteriostatic water per vial instructions.
Timing
Morning for energy benefits. Avoid late evening as it may interfere with sleep.
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Potential Side Effects
- Injection site stinging/burning (common)
- Nausea at higher doses
- Flushing
- Headache
- IV: chest tightness, cramping if infused too fast
Important Warnings
- IV infusions should only be administered by qualified medical personnel
- Subcutaneous injections commonly cause stinging — this is expected
Research References
- Yoshino J, et al. Cell Metab. 2018;27(3):513-528. doi:10.1016/j.cmet.2017.12.016
- Rajman L, et al. Cell Metab. 2018;27(3):529-547. doi:10.1016/j.cmet.2018.02.011
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Medical Disclaimer: This information is for educational purposes only and is not intended as medical advice. NAD+ may be regulated differently in various jurisdictions. Always consult with a qualified healthcare provider before beginning any peptide protocol. PepStack Pro does not sell peptides or provide medical advice. Content reviewed by Dr. Seth Miller, MD.